Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | No |
Product | DMEM/F12-KSR Baharvand et al. 2008 |
Baharvand, H., Hashemi, S. M., & Shahsavani, M. (2008). Differentiation of human embryonic stem cells into functional hepatocyte‐like cells in a serum‐free adherent culture condition. Differentiation, 76(5), 465-477. In this study, the use of a multistep protocol is presented with growth factors in a serum-free adherent culture configuration to mediate the hepatocyte differentiation of human ESCs (hESCs). The differentiated cells exhibited characteristic hepatocyte morphology, ultrastructure, and expressed hepatic-related genes as shown by reverse transcription-polymerase chain reaction and displayed antibody detectable expression of markers specific for hepatic maturation. These hepatocyte-like cells also demonstrated evidence of albumin and alpha-fetoprotein secretion, glycogen storage, urea production, uptake of low-density lipoprotein, and indocyanine green. The authors propose that the hepatocyte-like cells derived from hESCs by the present method may provide a useful model for the studies of key events during early liver development and a potential source of drug screening and transplantable cells for cell-replacement therapies. The formulation of the DMEM/F12-KSR is listed in the Materials and Methods under the heading; Culturing of hESCs. KSR is a commercial product. The precise formulation is proprietary. https://ac.els-cdn.com/S030146810960089X/1-s2.0-S030146810960089X-main.pdf?_tid=3c2ada30-e0f0-484f-b604-2a818e4b4039&acdnat=1534406015_b597909a62b366c1fece11cf2d2219d5 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |