Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | No |
Product | hES cell medium |
Baharvand, H., Totonchi, M., Taei, A., Seifinejad, A., Aghdami, N., & Salekdeh, G. H. (2009). Human-induced pluripotent stem cells: derivation, propagation, and freezing in serum-and feeder layer-free culture conditions. In Human Embryonic Stem Cell Protocols (pp. 425-443). Humana Press. In this study, the detailed methods for the generation of undifferentiated human iPS (hiPS) cells in feeder layer- and serum-free conditions are described. This system eliminates direct contact of stem cells with MEFs and reduces use of unknown serum factors that may have undesired activities and enables consistency in large-scale and long-term expansion of undifferentiated hiPS cells. The findings greatly simplify the method for induction of pluripotency and bring it one step closer to clinical applications. Moreover, the established hiPS cells showed chromosomal stability during long-term culture. hES cell medium consists of DMEM/F12 containing 20% KOSR, 2mM L-Gln, 0.1 mM b-mercaptoethanol, 1 mM (1%) nonessential amino acid, 100 units/ml penicillin and 100 mg/ml streptomycin, ITS, and 100 ng/ml basic-fibroblast growth factor. https://link.springer.com/content/pdf/10.1007%2F978-1-60761-369-5_23.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |