Hannoun, Z., Fletcher, J., Greenhough, S., Medine, C., Samuel, K., Sharma, R., ... & Iredale, J. P. (2010). The comparison between conditioned media and serum-free media in human embryonic stem cell culture and differentiation.
doi: 10.1089/cell.2009.0099
In this study, the authors initiated the process of standardizing hESC tissue culture and have employed two serum-free media: mTeSR (MT) and Stem Pro (SP). hESCs were maintained in a pluripotent state, for over 30 passages using MT and SP. Additionally, we present evidence that hESCs maintained in MT and SP generate equivalent levels of human hepatic endoderm as observed with mouse embryonic fibroblasts conditioned medium (MEF-CM). This data suggests that MT and SP are effective replacements for MEF-CM in hESC culture, contributing to the standardization of hESC in vitro models and ultimately their application.
mTeSR and Stem Pro are commercially available products. Their precise formulation is proprietary.
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