Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | VN:IGF-I chimera-containing medium |
Manton, K., Richards, S., Van Lonkhuyzen, D., Cormack, L., Leavesley, D., & Upton, Z. (2010). A chimeric vitronectin: IGF-I protein supports feeder-cell-free and serum-free culture of human embryonic stem cells. Stem cells and development, 19(9), 1297-1305. The aim of this study was to determine whether hES cells can be serially propagated in feeder-cell-free and serum-free conditions using medium containing our novel chimeric VN:IGF-I protein. Here is demonstrated that hES cells can be serially propagated and retain their undifferentiated state in vitro for up to 35 passages in our feeder-cell-free, serum-free, chemically defined media. This study demonstrates that the novel, fully defined, synthetic VN:IGF-I chimera-containing medium described herein is a viable alternative to media containing serum, and that in conjunction with laminin-coated plates facilitates feeder-cell-free and serum-free growth of hES. The formulation of the VN:IGF-I chimera-containing medium is listed in the Materials and Methods, under the heading; hES feeder-cell-free and serum-free culture. https://www.ncbi.nlm.nih.gov/pubmed/20128657 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |