Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | No |
Product | SFD medium |
Outten, J. T., Cheng, X., Gadue, P., French, D. L., & Diamond, S. L. (2011). A high-throughput multiplexed screening assay for optimizing serum-free differentiation protocols of human embryonic stem cells. Stem cell research, 6(2), 129-142. In this study, a high-throughput, 96-well plate, four-color flow cytometry-based assay was developed to optimize differentiation media cocktails and to screen a variety of conditions. The authors were able to differentiate hESCs to all three primary germ layers, screen for the effect of a range of activin A, BMP4, and VEGF concentrations on endoderm and mesoderm differentiation, and perform RNA-interference (RNAi)-mediated knockdown of a reporter gene during differentiation. The formulation of SFD medium is listed in the Materials and Methods, under the heading; hESC differentiation and summarized in Table 1 Differentiation media. https://ac.els-cdn.com/S1873506110001388/1-s2.0-S1873506110001388-main.pdf?_tid=4710d42f-beb7-4059-b9c9-5842230046da&acdnat=1533631036_4abdeb4c4c83284bf5c0b37a71fd04b0 |
|
Source | Literature - own formulation |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |