Cell line/type | ESCs (Embryonic Stem Cells) |
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Species | Human |
Animal free | Yes |
Product | TeSR2 Stephenson et al. 2012 |
Ilic, D., Stephenson, E., Wood, V., Jacquet, L., Stevenson, D., Petrova, A., ... & Cornwell, G. (2012). Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions. Cytotherapy, 14(1), 122-128. In this study, protocols were developed for the successful derivation of two normal and three specific mutation-carrying (Huntington's disease and myotonic dystrophy 1) genomically stable hES cell lines, and their adaptation to feeder-free culture, all under xeno-free conditions. The growth of five hES cell lines were tested on native decellularized HFF ECM in the presence of two commercially available xeno-free culture media, KO-SR XF and TeSR2. Presence of pluripotency markers and ability of cells to differentiate into derivatives of three germ layers, demonstrated that native decellularized HFF ECM and TeSR2 was a superb combination for maintaining the undifferentiated phenotype of all the hES lines tested. KCL-12 and KCL-13 SMC hES cell lines cultured on a native decellularized HFF ECM in the presence of KO-SR XF were prone to lower cell attachment and spontaneous cell differentiation, whereas in the presence of TeSR2 we had no such difficulties. TeSR2 is a commercially available, xeno-free, defined, feeder-free medium for maintenance of undifferentiated human ES and iPS cells. The precise formulation is proprietary. https://www.sciencedirect.com/science/article/pii/S1465324912706217 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |