Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | hESC differentiation medium |
Bai, H., Gao, Y., Arzigian, M., Wojchowski, D. M., Wu, W. S., & Wang, Z. Z. (2010). BMP4 regulates vascular progenitor development in human embryonic stem cells through a smad‐dependent pathway. Journal of cellular biochemistry, 109(2), 363-374. In this study, the roles of BMP on vascular progenitor development from human embryonic stem cells (hESCs) was investigated. Due to the low proliferation potential in terminal differentiated cells, it was attempted to characterize vascular progenitor differentiation in hESCs. Three serum-free media were formulated to generate vascular progenitor cells from hESCs, hESC-ECs, and hESC-SMCs, respectively. The new formulated serum-free media allowed us to investigate factors that direct hESC differentiation into vascular cells without interference from factors derived from sera. To induce hESC differentiation, undifferentiated hESCs on Hs27 feeder cells for 10 days were cultured in serum-free hESC differentiation medium, with or without growth factors. In a serum-free condition, hESCs sequentially differentiated into CD34+CD31-, CD34+CD31+, and then CD34-CD31+ cells during vascular cell development. The formulation of hESC differentiation medium, EC medium, and SMC medium are listed in Supplementary Table 1. Serum-free cell culture media. https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcb.22410 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |