Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | Vegeta-Cell medium |
Kunova, M., Matulka, K., Eiselleova, L., Trckova, P., Hampl, A., & Dvorak, P. (2010). Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells. Reproductive biomedicine online, 21(5), 676-686. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy. The formulation of Vegeta-Cell medium is listed in the Results, under the heading; Development and optimization of human ESC culture in humanized medium with VegetaCell. https://www.sciencedirect.com/science/article/pii/S1472648310004360 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |