Cell line/type | ESCs (Embryonic Stem Cells) |
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Species | Human |
Animal free | Yes |
Product | SBX medium |
Peiffer, I., Barbet, R., Zhou, Y. P., Li, M. L., Monier, M. N., Hatzfeld, A., & Hatzfeld, J. A. (2008). Use of xenofree matrices and molecularly-defined media to control human embryonic stem cell pluripotency: effect of low physiological TGF-β concentrations. Stem cells and development, 17(3), 519-534. In this study, it is demonstrated that matrices derived from human mesenchymal stem cells (hMSCs) can advantageously replace murine embryonic fibroblasts (MEF) or hMSC feeders. Moreover, a xenofree molecularly-defined SBX medium, containing a synthetic lipid carrier instead of albumin, can replace SR medium.The number of selected differentiation genes expressed by hESCs in these culture conditions was significantly lower than those expressed on MEF feeders in SR medium. In SBX, the positive effect of a nonphysiological concentration of activin A (10–30 ng/mL) to reduce differentiation during self-renewal could also be obtained by physiological concentrations of TGF- (100–300 pg/mL). In contrast, these TGF- concentrations added to activin favored differentiation as previously observed with TGF- concentrations of 1 ng/mL or more. Compared to SR-containing medium, SBX medium promoted down-regulation of CER1 and LEFTIES and up-regulation of GREM1. SBX medium is a commercial product. The formulation is proprietary. https://www.liebertpub.com/doi/pdf/10.1089/scd.2007.0279 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |