Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | No |
Product | StemPro hESC SFM |
Swistowski, A., Peng, J., Han, Y., Swistowska, A. M., Rao, M. S., & Zeng, X. (2009). Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them. PloS one, 4(7), e6233. In this study, a four-step scalable process that is readily transferable to a Good Manufacture Practice (GMP) facility for the production of functional dopaminergic neurons from hESCs for potential clinical uses is reported. Each of the steps (propagation of ESC-->generation of neural stem cells (NSC)-->induction of dopaminergic precursors-->maturation of dopaminergic neurons) could utilize xeno-free defined media and substrate, and cells could be stored at intermediate stages in the process without losing their functional ability. hESCs and NSCs can be maintained in xeno-free defined media for a prolonged period of time while retaining their ability to differentiate into authentic dopaminergic neurons. StemPro hESC SFM is based on commercial products, the precise formulation is not entirely known. The composition of StemPro hESC SFM is listed in the Materials and Methods, under the heading; Cell culture. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705186/pdf/pone.0006233.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Antibiotics free > Yes | Yes |
Contains phenol red > Yes | Yes |