Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | Unspecified |
Product | StemPro/mTeSR1 media |
Chin, A. C. P., Padmanabhan, J., Oh, S. K. W., & Choo, A. B. H. (2009). Defined and serum-free media support undifferentiated human embryonic stem cell growth. Stem cells and development, 19(6), 753-761. In this study, four commercially available serum-free and defined culture media were tested on 2 human embryonic stem cell (hESC) lines. For hESC cultured with defined StemPro and mTeSR1 media, the cells were maintained feeder-free on culture dishes coated with extracellular matrices (ECMs) with no requirement of feeder-conditioned media (CM). The data suggested that StemPro and mTeSR1 media were more optimal for hESC proliferation compared to cells grown on CM because the growth rate of hESC increased by 30%-40%, higher split ratio was thus required for weekly passaging. This is advantageous for the large-scale cultivation of hESC required in clinical applications. StemPro and mTeSR1 media are commercial products. Their formulation is proprietary. https://www.liebertpub.com/doi/pdf/10.1089/scd.2009.0210 |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |