|Cell line/type||ESCs (Embryonic Stem Cells)|
|Product||mTeSR1 Salvagiotto et al. 2011|
Salvagiotto, G., Burton, S., Daigh, C. A., Rajesh, D., Slukvin, I. I., & Seay, N. J. (2011). A defined, feeder-free, serum-free system to generate in vitro hematopoietic progenitors and differentiated blood cells from hESCs and hiPSCs. PloS one, 6(3), e17829.
In this study, to efficiently differentiate pluripotent cells into a pure population of hematopoietic progenitors, the authors have developed a new 2-dimentional, defined and highly efficient protocol that avoids the use of feeder cells, serum or embryoid body formation. The results show that a single matrix protein in combination with growth factors and a hypoxic environment is sufficient to generate from pluripotent cells hematopoietic progenitors capable of differentiating further in mature cell types of different lineages of the blood system. For potential clinical applications of cells generated with the described method, it was showed that the system can be easily converted to xenogenic-free conditions, as the only reagents of non-human origin used for our differentiation protocol
mTeSR1 medium is a commercial product. The precise formulation is proprietary.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060827/pdf/pone.0017829.pdf
|Chemically defined > Yes||Yes|