Salvagiotto, G., Burton, S., Daigh, C. A., Rajesh, D., Slukvin, I. I., & Seay, N. J. (2011). A defined, feeder-free, serum-free system to generate in vitro hematopoietic progenitors and differentiated blood cells from hESCs and hiPSCs. PloS one, 6(3), e17829.
DOI: 10.1371/journal.pone.0017829

In this study, to efficiently differentiate pluripotent cells into a pure population of hematopoietic progenitors, the authors have developed a new 2-dimentional, defined and highly efficient protocol that avoids the use of feeder cells, serum or embryoid body formation. The results show that a single matrix protein in combination with growth factors and a hypoxic environment is sufficient to generate from pluripotent cells hematopoietic progenitors capable of differentiating further in mature cell types of different lineages of the blood system. For potential clinical applications of cells generated with the described method, it was showed that the system can be easily converted to xenogenic-free conditions, as the only reagents of non-human origin used for our differentiation protocol
are the bFgf of zebrafish origin in the differentiation medium, and bovine serum albumin in serum replacer and in the pluripotent cell growth medium (mTeSR1), which can be substituted with the human counterparts.

mTeSR1 medium is a commercial product. The precise formulation is proprietary.