Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | No |
Product | Basal CM |
Koay, E. J., & Athanasiou, K. A. (2009). Development of serum-free, chemically defined conditions for human embryonic stem cell–derived fibrochondrogenesis. Tissue Engineering Part A, 15(8), 2249-2257. This study established serum-free, chemically defined conditions to generate fibrocartilage with human embryonic stem cells (hESCs). This study demonstrates that serum-free methods can be used during chondrogenic differentiation and cartilage tissue engineering with hESCs. Furthermore, this study demonstrates significant effects of TGF-b1 on the self-assembly of the serum-free standard (1 ng=mL of TGF-b1 in CM during differentiation) while also showing that cells differentiated under the serum-free and serum-based methods have a different response to growth factors during self-assembly. All three phases involved the use of a basal chondrogenic medium (CM) composed of high-glucose Dulbecco’s modified Eagle medium supplemented with various components. The formula is listed in the Methods. https://www.ncbi.nlm.nih.gov/pubmed/19231974 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |