|Cell line/type||ESCs (Embryonic Stem Cells)|
Koay, E. J., & Athanasiou, K. A. (2009). Development of serum-free, chemically defined conditions for human embryonic stem cell–derived fibrochondrogenesis. Tissue Engineering Part A, 15(8), 2249-2257.
This study established serum-free, chemically defined conditions to generate fibrocartilage with human embryonic stem cells (hESCs). This study demonstrates that serum-free methods can be used during chondrogenic differentiation and cartilage tissue engineering with hESCs. Furthermore, this study demonstrates significant effects of TGF-b1 on the self-assembly of the serum-free standard (1 ng=mL of TGF-b1 in CM during differentiation) while also showing that cells differentiated under the serum-free and serum-based methods have a different response to growth factors during self-assembly. All three phases involved the use of a basal chondrogenic medium (CM) composed of high-glucose Dulbecco’s modified Eagle medium supplemented with various components.
The formula is listed in the Methods.
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > Yes||Yes|