Cell line/type | PSCs (Pluripotent Stem Cells) |
---|---|
Species | Mouse |
Animal free | No |
Product | PSC differentiation medium |
Verma, I., Rashid, Z., Sikdar, S. K., & Seshagiri, P. B. (2017). Efficient neural differentiation of mouse pluripotent stem cells in a serum-free medium and development of a novel strategy for enrichment of neural cells. International Journal of Developmental Neuroscience, 61, 112-124. In this study, efficient differentiation of mouse PSCs into neural lineage was achieved, in the absence of exogenous molecules, by employing a serum-free culture medium containing knockout serum replacement (KSR). Embryoid bodies (EBs) cultured in this medium predominantly produced neural cells which included neural progenitors (15-18%), immature neurons (8-24%), mature neurons (10-26%), astrocytes (27-61%), and oligodendrocytes (approximately 1%). Different neuronal subtypes including glutamatergic, GABAergic, cholinergic, serotonergic, and dopaminergic neurons were generated. Importantly, neurons generated in the KSR medium were electrically active. This study provides the evidence that the KSR medium is ideal for the rapid and efficient generation of neural cells, including functional neurons, from PSCs without the requirement of any other additional molecule. Formulation of the PSC differentiation medium is listed in the Materials and methods, under the heading; 2.1. Culture and differentiation of PSCs. Knockout serum replacement (KSR) is a commercial product. Its formulation is proprietary. https://www.sciencedirect.com/science/article/pii/S0736574817301648?via%3Dihub |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |
Contains phenol red > Yes | Yes |