|Cell line/type||PSCs (Pluripotent Stem Cells)|
Uenishi, G., Theisen, D., Lee, J. H., Kumar, A., Raymond, M., Vodyanik, M., ... & Slukvin, I. (2014). Tenascin C promotes hematoendothelial development and T lymphoid commitment from human pluripotent stem cells in chemically defined conditions. Stem cell reports, 3(6), 1073-1084.
This study presents completely chemically defined conditions that are free of serum/serum components and animal proteins and are capable of supporting the scalable production of HE and definitive blood cells from hPSCs. This differentiation system will enable the precise interrogation of signaling molecules implicated in hematopoietic differentiation and provides a platform for producing cGMP-grade blood cells for clinical application.
The formulation of the medium can be found in the supplemental information, supplementary Table S1. Complete Content of IF9S medium.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263995/pdf/main.pdf
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > Yes||Yes|
|Antibiotics free > Yes||Yes|