STEMdiff™ APEL™ 2-LI Medium is a fully defined, low-insulin (LI), serum-free and animal component-free medium for the differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. It is based on the APEL formulation published by Dr. Andrew Elefanty and lacks undefined components such as protein-free hybridoma medium.

STEMdiff™ APEL™ 2-LI can be used in adherent or embryoid body (EB)-based protocols, such as with AggreWell™. Appropriate induction factors must be added before use. The low insulin content of this medium makes it particularly useful for differentiation to lineages in which insulin is a known inhibitor, such as cardiomyocyte.