Cell line/type | PSCs (Pluripotent Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | E8 medium Higuchi et al. 2015 |
Higuchi, A., Kao, S. H., Ling, Q. D., Chen, Y. M., Li, H. F., Alarfaj, A. A., ... & Hsu, S. T. (2015). Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity. Scientific reports, 5, 18136. In this study, hPSC cultures were investigated on biomaterials with different elasticities that were grafted with different nanosegments. Then, long-term culture (20 passages) of hPSCs was evaluated on one of the optimized PVA-oligoVN hydrogels (PVA-24h-1000) and Synthemax II dishes using the xeno-free culture medium Essential 8 (xeno-free medium) and compared to hPSCs cultured on Matrigel. The hPSCs cultured on the stiffest substrates (30.4 kPa) tended to differentiate after five days of culture, whereas the hPSCs cultured on the optimal elastic substrates (25 kPa) maintained their pluripotency for over 20 passages under xeno-free conditions. These results indicate that cell culture matrices with optimal elasticity can maintain the pluripotency of hPSCs in culture. The formulation of Essential 8 medium and the protocol for its preparation is listed in a previous article, Sharma et al. 2015. Sharma, A., Li, G., Rajarajan, K., Hamaguchi, R., Burridge, P. W., & Wu, S. M. (2015). Derivation of highly purified cardiomyocytes from human induced pluripotent stem cells using small molecule-modulated differentiation and subsequent glucose starvation. Journal of visualized experiments: JoVE, (97). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4677349/pdf/srep18136.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > Yes | Yes |
Contains phenol red > No | No |