Cell line/type | PSCs (Pluripotent Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | HFF-CM |
Abbasalizadeh, S., Larijani, M. R., Samadian, A., & Baharvand, H. (2012). Bioprocess development for mass production of size-controlled human pluripotent stem cell aggregates in stirred suspension bioreactor. Tissue Engineering Part C: Methods, 18(11), 831-851. In this study, the human foreskin fibroblast-conditioned medium (HFF-CM) instead of mouse embryonic fibroblast conditioned medium (MEF-CM) was examined for maintenance and culture of cell lines in xeno-free suspension culture. Eight different xeno-free conditioned and/or defined hPSC culture media combined with ROCKi treatment were screened for their effectiveness in supporting proliferation and self-renewal of the Royan H5 and hiPSC1 lines in static suspension culture. These media included Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) in the presence of L-Gln or GlutaMAX (Gibco-Invitrogen; 31331-028), neurobasal (Gibco-Invitrogen; 21103-049), StemPro (Gibco-Invitrogen; A10006-01) in the presence of L-Gln or GlutaMAX, and mTeSR (Stemcell Technologies; 05850). Every 7 days, hESC and hiPSC aggregates in the different medium groups were passaged and transferred to new plates that contained the same medium. The effectiveness of each medium was compared by cell counts, aggregate diameters, and viability measurements after a minimum of four passages. The cells underwent proliferation over multiple passages in the demonstrated xeno-free dynamic suspension culture while maintaining their self-renewal capabilities, as determined by marker expressions and in vitro spontaneous differentiation. For both cell lines, cell proliferation rates after four passages were significantly higher in CM of DMEM/F12 supplemented with L-Gln or GlutaMAX, Neurobasal medium, and mTeSR compared with the other groups (p<0.05). The formulation and protocol for preparation of HF-CM medium is listed in the Appendix, Supplementary Materials and Methods, under the heading; Maintenance of human embryonic stem cells and human induced pluripotent stem cells in static microcarrier-free suspension culture. https://www.liebertpub.com/doi/full/10.1089/ten.tec.2012.0161 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |