Cell line/type | PSCs (Pluripotent Stem Cells) |
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Species | Human |
Animal free | Yes |
Product | FTDA |
Frank, S., Zhang, M., Schöler, H. R., & Greber, B. (2012). Small molecule-assisted, line-independent maintenance of human pluripotent stem cells in defined conditions. PloS one, 7(7), e41958. In this study, 20 potential media ingredients were systematically evaluated for a defined hPSC culture system. Only components that reproducibly gave beneficial effects were ultimately combined to yield a simple and cost-effective formulation termed FTDA. This xeno-free medium is based on mimicking self-renewal factor activities present in mouse embryonic fibroblast-conditioned medium, at minimal dosages. Additionally, small molecule inhibitors of BMP and WNT signaling served to specifically suppress typical types of spontaneous differentiation seen in hPSC cultures. FTDA medium was suitable for the generation of human induced pluripotent stem cells and enabled robust long-term maintenance of diverse hPSC lines including hard-to-grow ones. Comparisons with existing defined media suggested reduced spontaneous differentiation rates in FTDA. The results imply that using supportive factors at minimal concentrations may still promote robust self-renewal and preserve pluripotency of hPSCs. The formulation of FTDA medium is listed in Table 1. Table 1. Summary of media ingredients tested provides a list of all included ingredients, optional ingredients, and excluded ingredients of FTDA medium. Table 1 also describes the beneficial effects of the supplements. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3408405/pdf/pone.0041958.pdf |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |