Cell line/type | PSCs (Pluripotent Stem Cells) |
---|---|
Species | Human |
Animal free | Unspecified |
Product | mTeSR1 Lu et al. 2012 |
Lu, H. F., Narayanan, K., Lim, S. X., Gao, S., Leong, M. F., & Wan, A. C. (2012). A 3D microfibrous scaffold for long-term human pluripotent stem cell self-renewal under chemically defined conditions. Biomaterials, 33(8), 2419-2430. In this study, an engineered 3D microfiber system was reported that efficiently supports long-term hPSCs self-renewal under chemically defined conditions. The unique feature of this system lies in the application of a 3D ECM-like environment in which cells are embedded, that affords: (i) uniform high cell loading density in individual cell-laden constructs (w107 cells/ml); (ii) quick recovery of encapsulated cells (<10 min at 37 C) with excellent preservation of cell viability and 3D multicellular structure; (iii) direct cryopreservation of the encapsulated cells in situ in the microfibers with >17-fold higher cell viability compared to those cultured on Matrigel surface; (iv) long-term hPSC propagation under chemically defined conditions. The used culture medium, mTeSR1 medium, is a commercial product. The precise formulation is proprietary. https://ac.els-cdn.com/S0142961211014311/1-s2.0-S0142961211014311-main.pdf?_tid=db155442-0880-4b80-93af-de667922cd22&acdnat=1530275091_0421822cad6708b79e62fd72432a3b25 |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |