|Cell line/type||Hepatocytes (general)|
Bowen, W. C., Michalopoulos, A. W., Orr, A., Ding, M. Q., Stolz, D. B., & Michalopoulos, G. K. (2014). Development of a chemically defined medium and discovery of new mitogenic growth factors for mouse hepatocytes: mitogenic effects of FGF1/2 and PDGF. PloS one, 9(4), e95487.
The work presented in this manuscript describes a chemically defined serum-free medium that allows growth of mouse hepatocytes in primary culture and high response to growth factors comparable to those seen with the rat.
The formulation of HGM medium has been previously described and was the basis of modifications for the formulation of the MHGM medium. The formula of both HGM and MHGM is listed in the Materials and Methods, under the heading Formulation of the HGM (Hepatocyte Growth Medium) and MHGM (Mouse Hepatocyte Growth Medium).https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990636/pdf/pone.0095487.pdf
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > No||No|