Cell line/type | Hepatocytes (general) |
---|---|
Species | Human |
Animal free | Yes |
Product | Modified Williams E medium |
Watanabe, M., Zemack, H., Johansson, H., Hagbard, L., Jorns, C., Li, M., & Ellis, E. (2016). Maintenance of hepatic functions in primary human hepatocytes cultured on xeno-free and chemical defined human recombinant laminins. PloS one, 11(9), e0161383. In this study, the distribution of laminin isoforms in human liver tissue and isolated primary human hepatocytes by western blot analysis was determined, and the efficacy of different human recombinant laminin isoforms on hepatic functions during culture was investigated. A defined culture system, free of animal components was used. The authors conclude that all forms of human recombinant laminin tested maintain cell viability and liver-specific functions of primary human hepatocytes, and that recombinant laminin is a promising xeno-free and chemical defined strategy for preservation of hepatocyte specific function in vitro. Williams E medium was supplemented with different components as specified in the Materials and Methods, under the heading; Cell culture. The formulation of Williams E medium can be found online--> https://www.sigmaaldrich.com/life-science/cell-culture/learning-center/media-formulations/williams.html https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012698/pdf/pone.0161383.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |