Cell line/type | Hepatocytes (general) |
---|---|
Species | Porcine (Pig) |
Animal free | No |
Product | DMEM/199 medium |
Caperna, T. J., Blomberg, L. A., Garrett, W. M., & Talbot, N. C. (2011). Culture of porcine hepatocytes or bile duct epithelial cells by inductive serum-free media. In Vitro Cellular & Developmental Biology-Animal, 47(3), 218-233. In this study, a serum-free, feeder cell-dependent, selective culture system for the long-term culture of porcine hepatocytes or cholangiocytes was developed. This was accomplished by the co-culture of near-single-cell suspensions of partially purified, freshly isolated, pig hepatocytes on feeder layers of mitotically inactivated STO fibroblasts. The differentiation of the isolated pig liver cells into hepatocyte monolayers or bile ductules was dictated by the absence or presence of DEX combined with a higher or lower level of DMSO in the serum-free medium. It was demonstrated that the isolation of hepatocyte-enriched porcine liver cells, which can be maintained for several weeks as differentiated hepatocytes or, under appropriate culture conditions, can be induced to differentiate into bile ductules. The cells were cultured in serum-free DMEM/199 medium supplemented with different components as specified in the Materials and Methods, under the heading; Cell culture. https://link.springer.com/content/pdf/10.1007%2Fs11626-010-9382-3.pdf |
|
Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |