Cell line/type | Hepatocytes (general) |
---|---|
Species | Porcine (Pig) |
Animal free | Yes |
Product | Williams E medium Rasmussen et al. 2017 |
Rasmussen, M. K. (2017). Induction of cytochrome P450 mRNA in porcine primary hepatocytes cultured under serum free conditions: Comparison of freshly isolated cells and cryopreserved. Experimental cell research, 360(2), 218-225. In this study, the impact of serum in the culture medium of porcine primary hepatocytes (PPH) was investigated on markers of dedifferentiation as well as the impact on CYP induction. The effects were studied in both freshly isolated primary hepatocytes as well as cryopreserved. The exclusion of serum in the culturing media were not introducing significant dedifferentiation as judged by the gene expression of alpha-fetoprotein, albumin, glucose-6-phosphatase and the constitutive expression of selected transcription factors and CYP. The induction of CYP2B22 and CYP3A29 by phenobarbital and rifampicin, were greater in hepatocytes cultured without serum. The same were not observed for TCDD induced CYP1A2 expression. In conclusion, PPH cultured under serum-free conditions results in little or no dedifferentiation, while being more responsive to known CYP inducers. Hence, it can be suggested that PPH cultured under serum-free conditions provides a reliable hepatocyte model to investigate CYP gene regulation. The cells were cultured in serum-free serum-free Williams E medium. Williams E medium was supplemented with different components as specified in the Materials and Methods, under the heading, 2.5. Cell culturing and treatment with inducers. https://www.sciencedirect.com/science/article/pii/S0014482717304895 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Unspecified | Optional |
Antibiotics free > No | No |