Cell line/type | MDCK (canine kidney cell) |
---|---|
Species | Canine (Dog) |
Animal free | Yes |
Product | Plus™ MDCK |
Hu, A. Y. C., Tseng, Y. F., Weng, T. C., Liao, C. C., Wu, J., Chou, A. H., ... & Hsiao, C. H. (2011). Production of inactivated influenza H5N1 vaccines from MDCK cells in serum-free medium. PloS one, 6(1), e14578. This study presents an improved high-yield manufacturing process for the inactivated influenza H5N1 vaccines using Madin-Darby canine kidney (MDCK) cells grown in a serum-free (SF) medium microcarrier cell culture system. SF medium (Plus MDCK) could support both the cell-growth in the solid microcarriers and H5N1 virus-replication in the well controlled and scalable bioreactors; the cell-based flu vaccines could be manufactured in a safe and user-friendly process with fewer contaminants and consistently high yield. Plus™ MDCK is a commercially available product. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025921/pdf/pone.0014578.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |