Cell line/type | MDCK (canine kidney cell) |
---|---|
Species | Canine (Dog) |
Animal free | No |
Product | MDCK-SFM |
Huang, D., Zhao, L., & Tan, W. (2011). Adherent and single-cell suspension culture of Madin-Darby canine kidney cells in serum-free medium. Sheng wu gong cheng xue bao= Chinese journal of biotechnology, 27(4), 645-652. In this study, a serum-free adherent culture process for MDCK cells was developed with an in-house prepared serum-free medium MDCK-SFM. A cell line named ssf-MDCK was derived, which was amenable for single-cell suspension culture in the serum-free medium. During serum-free batch culture of MDCK cells, the peak viable cell density and maximum specific growth rate were 3.81 x 10(6) cells/mL and 0.056 h(-1), respectively; 3.6- and 1.6-fold increase compared with those in serum-containing adherent batch culture. Growth and metabolic characteristics of MDCK cells were compared in serum-containing adherent culture, serum-free adherent culture and serum-free single-cell suspension culture. Less metabolic by-products were produced in both serum-free cultures. In serum-free single-cell suspension batch culture, the viable cell density was highest. These results are critical for establishing large-scale suspension culture of MDCK cells as subsequent well as large-scale influenza vaccine production. The precise formulation of the in-house prepared serum-free medium MDCK-SFM is patented. https://patents.google.com/patent/CN101760442B/en https://www.ncbi.nlm.nih.gov/pubmed/21848001 |
|
Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |