Cell line/type | MDCK (canine kidney cell) |
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Species | Canine (Dog) |
Animal free | Unspecified |
Product | SFM4BHK21 medium |
Van Wielink, R., Kant-Eenbergen, H. C. M., Harmsen, M. M., Martens, D. E., Wijffels, R. H., & Coco-Martin, J. M. (2011). Adaptation of a Madin–Darby canine kidney cell line to suspension growth in serum-free media and comparison of its ability to produce avian influenza virus to Vero and BHK21 cell lines. Journal of virological methods, 171(1), 53-60. In this study, a novel MDCK cell line (MDCK-SFS) is described that grows efficiently in suspension and retained high expression levels of both alpha-2,6 and alpha-2,3 sialic acid receptors, which bind preferably to human and avian influenza viruses, respectively. The production of avian influenza virus by BHK21, Vero and MDCK-SFS cell lines was compared. MDCK-SFS cells yielded the highest infectious virus titres and virus genome equivalent concentration for five of the eight influenza strains analyzed and the highest hemagglutination activity for all eight virus strains. Taken together with their suitability for suspension growth this makes the MDCK-SFS cell line potentially useful for large scale influenza virus production. Adaptation of cell lines was attempted in four SF cell culture media: Optipro, UltraMDCK, SFM4MegaVir (Hyclone) and SFM4BHK21 medium. MDCK-SF cells only grew in suspension in SFM4BHK21 medium. SFM4BHK21 is a prototype medium designed specifically for BHK21 cells. The formulation of SFM4BHK21 medium is proprietary, https://ac.els-cdn.com/S016609341000354X/1-s2.0-S016609341000354X-main.pdf?_tid=93c529a2-7d30-4e6e-8f58-1b108874bfdc&acdnat=1533624785_e252279495fcd75a1cada55d17de122e |
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Source | Literature - commercial |
Chemically defined > Unspecified | Unspecified |