Cell line/type HCE-T and HCK (corneal cells. HCK: RRID:CVCL_6F04)
Species Human
Animal free No
Product KGM

From: Hahne, M., & Reichl, S. (2011). Development of a serum-free human cornea construct for in vitro drug absorption studies: the influence of varying cultivation parameters on barrier characteristics. International journal of pharmaceutics, 416(1), 268-279.

In this study, the serum-free media KGM and Pan+ were the most promising. They were subsequently
used for cultivating the Hemicornea construct.

Compositions of these media:

Abbreviation Base medium Supplements
KGM Keratinocyte growth medium 30 g/mL bovine pituitary extract (BPE)
0.1 ng/mL human epidermal growth factor (hEGF)
0.5 g/mL hydrocortisone
5 g/mL insulin
0.5 mM calcium chloride
15 ng/mL amphotericin B
30 g/mL gentamicin sulfate

 

https://doi.org/10.1016/j.ijpharm.2011.07.004
Source Literature - modified commercial product
Chemically defined > Yes Yes

Hahne & Reichl (2011) - Hahne, M., & Reichl, S. (2011). Development of a serum-free human cornea construct for in vitro drug absorption studies: the influence of varying cultivation parameters on barrier characteristics. International journal of pharmaceutics, 416(1), 268-279.