Cell line/type | Corneal Stromal MSCs |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM-HPL Matthyssen et al. 2017 |
Matthyssen, S., Dhubhghaill, S. N., Van Gerwen, V., & Zakaria, N. (2017). Xeno-Free Cultivation of Mesenchymal Stem Cells From the Corneal Stroma. Investigative ophthalmology & visual science, 58(5), 2659-2665. This study investigated animal-free alternatives to FBS for cultivation of human corneal stromal MSCs. Proliferative capacity was studied for cultures supplemented with different concentrations (2.5%, 5%, and 10%) of FBS, human AB serum, human platelet lysate (HPL), and XerumFree. Unsupplemented basal medium was used as a control. HPL is the only consistent non-xeno supplement where hMSC cultures show significantly higher proliferation than the 10% FBS-supplemented cultures. Both FBS- and HPL-supplemented hMSC cultures showed plastic adherence and trilineage differentiation, and no significant differences were found in the expression of the hMSC marker panel. The authors conclude that HPL is the best supplement for expansion of human corneal stromal MSCs. HPL significantly outperforms human AB serum, the chemically defined XerumFree, and even the gold standard, FBS. The xeno-free nature of HPL additionally confers preferred standing for use in GMP-regulated clinical trials using human corneal stromal MSCs. The formulation of DMEM-HPL medium is specified in the Materials and Methods, under the heading; Media and Supplements. https://iovs.arvojournals.org/data/journals/iovs/936209/i1552-5783-58-5-2659.pdf |
|
Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |