From: Guo, X., Spradling, S., Stancescu, M., Lambert, S., & Hickman, J. J. (2013). Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1. Biomaterials, 34(18), 4418-4427.

To initiate sensory neuron differentiation, the medium was replaced with KSR medium that contained 10 mM SB43152 and 500 ng/ml Noggin. KSR mediumwas prepared by supplementing 800 ml Knockout DMEM (Invitrogen, Cat. 11330-032) with 150 ml KSR (knockout serum replacement, (Invitrogen, Cat. 10828-028), 10 ml LGlutamine (Invitrogen, Cat. 21051-016), 10 ml Penicillin/Streptomycin (100X, Invitrogen, Cat. 15070-063), 10 ml 10 mM MEM (100X, nonessential amino acids, Invitrogen, Cat. 11140-050) and 1 ml b-mercaptoethanol (1000X, Invitrogen, Cat. 21985-023). To feed the cells during differentiation, the medium was replaced and gradually switched from KSR medium to N2B medium (NeuralStem Inc) according to the following schedule: day 2 (75% KSR, 25% N2B), day 4 (50% KSR, 50% N2B), day 6 (25% KSR, 75% N2B), days 8 & 10 (0% KSR, 100% N2B). However, the content of SB43152 and Noggin (10 mM and 500 ng/ml, respectively) remained constant throughout the procedure.