Cell line/type | UCMSCs (umbilical cord mesenchymal stem cells) |
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Species | Human |
Animal free | Yes |
Product | MesenCult-XF Yang et al. 2012 |
Yang, T., Chen, G. H., Xue, S. L., Qiao, M., Liu, H. W., Tian, H., ... & Wu, D. P. (2012). Comparison of the biological characteristics of serum-free and fetal bovine serum-contained medium cultured umbilical cord-derived mesenchymal stem cells. Zhonghua xue ye xue za zhi= Zhonghua xueyexue zazhi, 33(9), 715-719. In this study, the objective was to compare the differences of biological characteristics between human umbilical cord-derived mesenchymal stem cells (UC-MSCs) cultured by serum-free medium or fetal bovine serum-contained complete medium to establish a xenogeneic protein-free UC-MSCs culture system. Healthy human umbilical cord segments were digested with collagenase. UC-MSCs were cultured by serum-free MesenCult-XF medium and FBS-based alphaMEM complete medium, then analyzed the morphology, immunophenotype, expansion potential, lineage differentiation potential, karyotype and immunosuppression of early passages. Compared with serum-contained medium cultured early passages of UC-MSCs, the cell diameter of serum-free medium cultured UC-MSCs was smaller with higher expansion potential. No xenogeneic proteins were presented in UC-MSCs preparations when cultured with serum-free medium. Human UC-MSCs suppressed T-cell proliferation in a dose-dependent manner. The immunosuppressive potential of serum-free medium cultured UC-MSCs was higher than FBS-based medium cultured ones. MesenCult-XF medium is a commercially available medium. The precise formulation is proprietary. http://www.bloodjournal.org/content/120/21/4737?sso-checked=true |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |