Cell line/type | UCMSCs (umbilical cord mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | StemPro® MSC SFM Mizukami et al. 2016 |
Mizukami, A., Fernandes‐Platzgummer, A., Carmelo, J. G., Swiech, K., Covas, D. T., Cabral, J. M., & da Silva, C. L. (2016). Stirred tank bioreactor culture combined with serum‐/xenogeneic‐free culture medium enables an efficient expansion of umbilical cord‐derived mesenchymal stem/stromal cells. Biotechnology journal, 11(8), 1048-1059. In this study, we firstly established a spinner flask culture system combining gelatin-based Cultispher((R)) S microcarriers and xeno-free culture medium for the expansion of umbilical cord matrix (UCM)-derived MSC. This system enabled the production of 2.4 (+/-1.1) x10(5) cells/mL (n = 4) after 5 days of culture, corresponding to a 5.3 (+/-1.6)-fold increase in cell number. The established protocol was then implemented in a stirred-tank bioreactor (800 mL working volume) (n = 3) yielding 115 million cells after 4 days. Upon expansion under stirred conditions, cells retained their differentiation ability and immunomodulatory potential. The development of a scalable microcarrier-based stirred culture system, using xeno-free culture medium that suits the intrinsic features of UCM-derived MSC represents an important step towards a GMP compliant large-scale production platform for these promising cell therapy candidates. Cells were cultured in commercially available StemPro® MSC SFM XenoFree culture medium. The precise formulation of this medium is proprietary. https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.201500532 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |