Cell line/type | UCMSCs (umbilical cord mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | a-MEM-AB HS |
Tozetti, P. A., Caruso, S. R., Mizukami, A., Fernandes, T. R., da Silva, F. B., Traina, F., ... & Swiech, K. (2017). Expansion strategies for human mesenchymal stromal cells culture under xeno‐free conditions. Biotechnology progress, 33(5), 1358-1367. In this study, successful expansion of umbilical cord matrix-derived MSC (UCM MSC) in microcarriers using AB human serum (AB HS) as a FBS alternative to enable a xeno-free scalable bioprocess was demonstrated. The cells were isolated and cultured in a-MEM culture medium supplemented with 1% Penicillin-Streptomycin, 2.6 g/L HEPES, 2.2 g/L of sodium bicarbonate and 15% AB HS The formulation of a-MEM-AB HS medium is specified in the Material and Methods, under the heading; Cells and culture medium. https://onlinelibrary.wiley.com/doi/pdf/10.1002/btpr.2494 |
|
Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |