Tozetti, P. A., Caruso, S. R., Mizukami, A., Fernandes, T. R., da Silva, F. B., Traina, F., ... & Swiech, K. (2017). Expansion strategies for human mesenchymal stromal cells culture under xeno‐free conditions. Biotechnology progress, 33(5), 1358-1367.
DOI: 10.1002/btpr.2494

In this study,  successful expansion of umbilical cord matrix-derived MSC (UCM MSC) in microcarriers using AB human serum (AB HS) as a FBS alternative to enable a xeno-free scalable bioprocess was demonstrated. The cells were isolated and cultured in a-MEM culture medium supplemented with 1% Penicillin-Streptomycin, 2.6 g/L HEPES, 2.2 g/L of sodium bicarbonate and 15% AB HS

The formulation of a-MEM-AB HS medium is specified in the Material and Methods, under the heading; Cells and culture medium.