Cell line/type | UCMSCs (umbilical cord mesenchymal stem cells) |
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Species | Human |
Animal free | Yes |
Product | CDM Wu et al. 2016 |
Wu, X., Kang, H., Liu, X., Gao, J., Zhao, K., & Ma, Z. (2016). Serum and xeno‐free, chemically defined, no‐plate‐coating‐based culture system for mesenchymal stromal cells from the umbilical cord. Cell proliferation, 49(5), 579-588. In this study, the authors report a serum-free, xeno-free, completely chemically defined and no-plate-coating-based culture system for the isolation and expansion of umbilical cord mesenchymal stromal cells (UCMSCs), whose biological characteristics were evaluated and compared with serum-containing medium (SCM) methods. The culture system described in this article could not only supported UCMSC primary cultures but also allowed for their expansion at low seeding density. Compared to SCM, UCMSCs in serum-free media (SFM) exhibited (i) higher proliferative and colony-forming capacities; (ii) distinctly different morphologies; (iii) similar phenotype; (iv) similar pluripotency-associated marker expression; (v) superior osteogenic, but reduced adipogenic differentiation capacitities. In addition, UCMSCs cultured in SFM retained similar immunomodulatory properties to those in SCM. The formula is listed in Table 1 The composition of chemically defined serum-free medium for UCMSCs. https://onlinelibrary.wiley.com/doi/pdf/10.1111/cpr.12279 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Wu et al (2016) - Wu, X., Kang, H., Liu, X., Gao, J., Zhao, K., & Ma, Z. (2016). Serum and xeno‐free, chemically defined, no‐plate‐coating‐based culture system for mesenchymal stromal cells from the umbilical cord. Cell proliferation, 49(5), 579-588. And: Wu, X., Ma, Z., Wu, D. (2020) Derivation of clinical-grade mesenchymal stromal cells from umbilical cord under chemically defined culture condition – platform for future clinical application. https://doi.org/10.1016/j.jcyt.2020.03.431 |
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Contains phenol red > No | No |
Wu et al (2016) - Wu, X., Kang, H., Liu, X., Gao, J., Zhao, K., & Ma, Z. (2016). Serum and xeno‐free, chemically defined, no‐plate‐coating‐based culture system for mesenchymal stromal cells from the umbilical cord. Cell proliferation, 49(5), 579-588. And: Wu, X., Ma, Z., Wu, D. (2020) Derivation of clinical-grade mesenchymal stromal cells from umbilical cord under chemically defined culture condition – platform for future clinical application. https://doi.org/10.1016/j.jcyt.2020.03.431 |
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Antibiotics free > Yes | Yes |