Panserin 401 is a fully defined ready-to-use medium for the serum-free cultivation of a multitude of adherent and non adherent cells.

Composition

Based on Iscove's MEM, with additional trace elements, albumin, cholesterol, soy lipids and vitamins. It does not contain any growth or attachment factors. Protein and animal-derived components < 0.2 % w/v. Hydrolysates < 0.001 % w/v.

Suitability

Panserin 401 is a multi-purpose medium suitable for a variety of cells. As the medium contains no growth factors there is a possibility to investigate the effects of specific growth factors added to the cell culture. Panserin 401 does not contain any attachment factors. With some cell types a pre-treatment of the cell culture vessels with gelatine, collagen, poly-D-lysine or fibronectin may support or enable a culture under serum-free conditions. Please note that a coating may be especially important with low seeding densities.

Among others the following cells have been cultivated successfully:

• Hybridoma
• Lymphocytes
• Macrophages
• Fibroblasts
• Melanocytes
• Carcinoma-cells
• HEK-cells
• HeLa-cells
• CHO-cells