|Cell line/type||Vero cells|
|Species||African green monkey|
Petiot, E., Fournier, F., Gény, C., Pinton, H., & Marc, A. (2010). Rapid screening of serum-free media for the growth of adherent Vero cells by using a small-scale and non-invasive tool. Applied biochemistry and biotechnology, 160(6), 1600-1615.
In this study, a new, rapid, and non-invasive technique is presented to specifically monitor cultures of adherent cells in 96-well plates. This study showed that an improved serum-free medium, with some component groups being removed or added, can be rapidly formulated to reach respectively similar or 1.6 times higher cell density than in the original medium. The results from this global approach could be helpful to further focus experiments on identified medium components.
The confidential serum-free culture medium used was entitled complete medium, MC. In this work, the improvement of the complete medium, MC, was based on the study of its composition effect on Vero cell growth. The original complete medium MC was developed several years ago by Sanofi pasteur to specifically cultivate Vero cells in vaccine production processes, but its formulation was not obtained by a similar screening method. Because of
|Source||Literature - modified commercial product|
|Chemically defined > Yes||Yes|