Krawczyk, K. M., Matak, D., Szymanski, L., Szczylik, C., Porta, C., & Czarnecka, A. M. (2018). Culture in embryonic kidney serum and xeno-free media as renal cell carcinoma and renal cell carcinoma cancer stem cells research model. Cytotechnology, 70(2), 761-782. 
DOI: 10.1007/s10616-017-0181-5

In this study,  animal-derived component free media were screened. Three xenofree defined media and stemcell dedicated media were tested, but only one, FreeStyle medium, significantly enhanced 2D growth of Renal cell cancer (RCC) cells and enabled to maintain their epithelial morphology. Moreover, FreeStyle medium supported suspension growth of human kidney cancer stem cells (HKCSC) and promoted round lymphoblast-like spherical morphology, as expected for these stem cells.

FreeStyle^TM 293 expression medium, an animal origin-free, chemically defined, serum-free, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension containing GlutaMAX^TM supplement. FreeStyle^TM 293 expression medium is a commercial product. The precise formulation is proprietary.