From: Radošević, K., Dukić, B., Andlar, M., Slivac, I., & Srček, V. G. (2016). Adaptation and cultivation of permanent fish cell line CCO in serum-free medium and influence of protein hydrolysates on growth performance. Cytotechnology, 68(1), 115-121.

In this study, the authors describe the adaptation of channel catfish ovary (CCO) cell line to commercially available Ultra Culture serum-free medium by gradual reduction of serum concentration from 10 to 0 %. DME medium supplemented with 10% FBS was used for initial expanding of CCO cells in T-flasks, while for the adaptation to serum-free conditions UltraCulture SFM, supplemented with 10–0 % FBS was used.

UltraCulture SFM is a commercial product, and consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The formulation for UltraCULTURE™ Medium has been submitted to the FDA as a Master File. Permission to cross-reference the Master File may be obtained by contacting the Regulatory Affairs Department.