Cell line/type | HEK293T |
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Species | Human |
Animal free | Yes |
Product | UltraCulture medium Kuroda et al. 2009 |
Kuroda, H., Kutner, R. H., Bazan, N. G., & Reiser, J. (2009). Simplified lentivirus vector production in protein-free media using polyethylenimine-mediated transfection. Journal of virological methods, 157(2), 113-121. This study describes optimized protocols for lentiviral vector production based on polyethylenimine (PEI)-mediated transfection, resulting in more consistent lentiviral vector stocks. To achieve this goal, simple production methods for high-titer lentiviral vector production involving transfection of HEK 293T cells immediately after plating were developed. Importantly, high titers were obtained with cell culture media lacking serum or other protein additives altogether. The cells were cultured in UltraCulture medium supplemented with different components as specified in the Materials and Methods, under the heading; 2.4. Lentiviral vector production in six-well plates. UltraCulture medium is a commercially available product. The precise formulation is proprietary. https://ac.els-cdn.com/S016609340800431X/1-s2.0-S016609340800431X-main.pdf?_tid=afb47fa5-2528-45da-96c9-7734ce54414c&acdnat=1534241959_6157106b2889bb3b27236f67f42f2ce0 |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |