Cell line/type | BMSCs (bone marrow derived stem cells) |
---|---|
Species | Rabbit |
Animal free | No |
Product | CDSFM |
Wu, W., Zhou, Y., & Tan, W. (2009). In vitro culture of bone marrow-derived mesenchymal stem cells in a chemically-defined serum-free medium. Sheng wu gong cheng xue bao= Chinese journal of biotechnology, 25(1), 121-128. This study is aimed to design a chemically-defined serum-free medium (CDSFM) to support in vitro culture of bone marrow-derived mesenchymal stem cells (BM-MSCs). BM-MSCs were isolated from the femoral bones of one-month-old New Zealand Rabbits with density gradient centrifugation. We compared the proliferation capability, cell cycle, colony-forming efficiency, osteogenic and adipogenic differentiation capabilities of BM-MSCs cultured in CDSFM with those cultured in serum-containing medium (SCM). After 10 days culture, BM-MSCs were expanded by 50 folds in CDSFM, while only 40 folds in SCM. The expanded BM-MSCs in CDSFM preserved differentiation potentials into mesenchymal lineages in vitro, including adipocytes and osteoblasts. We have designed a chemically-defined serum-free medium that could support in vitro proliferation and maintain the properties of BM-MSCs as stem cells, which could be applied to cell-based therapy and biomedical research. The formulation of CDSFM is listed in this article. The base medium of CDSFM is UltraCULTURE Medium. The formulation for UltraCULTURE Medium has been submitted to the FDA as a Master File. Permission to cross-reference the Master File may be obtained by contacting the Regulatory Affairs Department. https://www.ncbi.nlm.nih.gov/pubmed/19441237 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |