Cell line/type | P3X63Ag8.653, COS-1, COS-7, Vero, HEK 293, SP2/0-Ag14, BHK 21, NS0, HEP-1 |
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Species | Mammalian (general) |
Animal free | Yes |
Product | FILOCETH (cryopreservation) |
Hernández, Y. G., & Fischer, R. W. (2007). Serum-free culturing of mammalian cells-adaptation to and cryopreservation in fully defined media. ALTEX-Alternatives to animal experimentation, 24(2), 110-116. In this work, the authors compered existing cryo-media formulae, evaluate the optimal cooling rate and demonstrate the positive influence of Pluronic F68™ in the newly developedfreezing medium “FILOCETH” on the survival rate of cryopreserved cells after thawing. The low temperature conservation of serum independent cells was achieved with the newly developed fully defined freezing media FILOCETH. The FILOCETH freezing medium developed in this work has the following composition: 89% chemically defined, serum-free medium (TurboDoma) 10% dimethyl sulphoxide 1% Pluronic F68™. The formulation is patented. http://www.altex.ch/resources/altex_2007_2_110_116_GonzalezHernandez.pdf |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |