Cell line/type | SVF (stromal vascular fraction of adipose tissue) cells |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM-PVP Thirumala et al. 2010 (cryopreservation) |
Thirumala, S., Gimble, J. M., & Devireddy, R. V. (2010). Cryopreservation of stromal vascular fraction of adipose tissue in a serumāfree freezing medium. Journal of tissue engineering and regenerative medicine, 4(3), 224-232. The objective of this study was to test the hypothesis that the stromal vascular fraction (SVF) of adipose tissue can be effectively cryopreserved and stored in liquid nitrogen, using a freezing medium containing high molecular weight polymers, such as methylcellulose (MC) and/or polyvinylpyrollidone (PVP), as the cryoprotective agent (CPA) instead of DMSO. The percentage of viable cells obtained with 10% PVP and DMEM was comparable with that obtained in freezing medium with DMSO and serum (HS or FCS). The results suggest that post-thaw SVF cell viability and adipogenic and osteogenic differentiability can be maintained even when they are frozen in the absence of serum and DMSO but with 10% PVP in DMEM. The formulation of the serum-free DMEM-PVP based medium is listed in the Materials and Methods. https://onlinelibrary.wiley.com/doi/pdf/10.1002/term.232 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |