Cell line/type | ASCs (adipose tissue derived stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | AR8 Parker et al. 2007 |
Parker, A. M., Shang, H., Khurgel, M., & Katz, A. J. (2007). Low serum and serum-free culture of multipotential human adipose stem cells. Cytotherapy, 9(7), 637-646. In this study, the authors developed two new culture methods, a very low human serum expansion medium and a completely serum-free medium, in an effort to eliminate the use of animal products in human adipose stem cell (ASC) cultures. Through serial testing, a highly enriched medium formulation was developed for use with and without the addition of 0.5% human serum, an amount easily obtainable from autologous blood draws. Very low-serum culture yielded population-doubling times averaging 1.86 days in early passage, while the serum-free formulation was associated with less robust cell growth, with doubling times averaging 5.79 days. ASC in both conditions maintained its ability to differentiate into adipo-, chondro- and osteogenic lineages in vitro, despite lower expression of CD34 in early passage. Expression of ALDH, HLA, CD133, CD184, and CD31 was comparable with that seen in cells cultured in 10% FBS. These newly developed culture conditions provide a unique environment within which to study ASCs without the interference of animal serum, and allow for the rapid expansion of autologous ASCs in culture in an animal product-free environment for use in human clinical trials. The formulation of the AR8 medium is listed in Table 2. AR8 final formulation. AR8 can be used with and without the addition of 1/2% HS https://ac.els-cdn.com/S1465324907701317/1-s2.0-S1465324907701317-main.pdf?_tid=9a2db7b5-0c32-404f-a9c0-a1dc89ad9422&acdnat=1534421197_efbe6daeeac2438de787e13182b0be9e |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |