|Cell line/type||ASCs (adipose tissue derived stem cells)|
|Product||Sphere culture medium|
Dromard, C., Bourin, P., Andre, M., De Barros, S., Casteilla, L., & Planat-Benard, V. (2011). Human adipose derived stroma/stem cells grow in serum-free medium as floating spheres. Experimental cell research, 317(6), 770-780.
In this study, the authors have developed a new culture system that allows the expansion of ASC as spheres in a defined medium. These spheres can be passaged several times. They are not only aggregated cells but rather originate from single cells as clonal spheres can be obtained after seeding at very low density and reform clonal spheres after dissociation. These spheres can also revert to monolayer growth when plated in medium containing human plasma and even generate fibroblast-like colonies (CFU-f). Under several differentiation-specific media, spheres-derived ASC maintain their capacity to differentiate into osteoblasts, endothelial cells and adipocytes. These results indicate that human ASC can be maintained in a serum-free 3D culture system, which is of great interest for the expansion in bioreactors of autologous ASC and their use in clinical trials.
The formulation of the serum-free spere culture medium is listed in the Materials and Methods, under the heading; Cell culture.https://ac.els-cdn.com/S0014482711000036/1-s2.0-S0014482711000036-main.pdf?_tid=46a8c374-9438-48ca-a30e-a18c92f1b804&acdnat=1533722238_0269753e4d2906d09f234585251be3f4
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > Yes||Yes|
|Antibiotics free > No||No|