Escobar, C. H., & Chaparro, O. (2016). Xeno‐free extraction, culture, and cryopreservation of human adipose‐derived mesenchymal stem cells. Stem cells translational medicine, 5(3), 358-365.
DOI: 10.5966/sctm.2015-0094. 

In this study, after standardizing a human platelet lysate (hPL) production protocol, four human adipose tissue samples were processed through explants with fetal bovine serum (FBS)-supplemented or hPL-supplemented media for extracting the adipose-derived stem cells. The cells were cultivated in cell culture medium + hPL (5%) or FBS (10%). The explants cultured in hPL-supplemented media showed earlier and faster hASC proliferation than did those supplemented with FBS. Likewise, cells grown in hPL-supplemented media showed a greater proliferation rate, without losing the immunophenotype. Osteogenic differentiation of xeno-free hASC was higher than the hASC produced in standard conditions. However, adipogenic differentiation was reduced in xeno-free hASC. Finally, the cells cryopreserved in an hPL-based solution showed a higher cellular viability than the cells cryopreserved in an FBS-based. 

CM-hPL medium consists of  Opti-MEM  supplemented with penicillin, streptomycin, neomycin  and 5% hPL as specified in the Materials and Methods, under the heading; Extraction of hASCs.