|Cell line/type||ASCs (adipose tissue derived stem cells)|
|Product||StemPro MSC SFM XenoFree +CELLstart|
Yang, S., Pilgaard, L., Chase, L. G., Boucher, S., Vemuri, M. C., Fink, T., & Zachar, V. (2012). Defined xenogeneic-free and hypoxic environment provides superior conditions for long-term expansion of human adipose-derived stem cells. Tissue Engineering Part C: Methods, 18(8), 593-602.
In this study, the authors aimed to assess the benefits of maintaining cultures of adipose-derived stem cells (ASCs) in a defined culture system devoid of xenogeneic components (xeno-free) and hypoxia over a 49-day growth period. the obtained data provide evidence that conditions involving StemPro mesenchymal stem cells serum-free medium (SFM) Xeno-Free and hypoxia (5% oxygen concentration) in the culture atmosphere provide a superior proliferation rate compared to a standard growth environment comprised of alpha-modified Eagle medium (A-MEM) supplemented with fetal calf serum (FCS) and ambient air (20% oxygen concentration) or that of A-MEM supplemented with FCS and hypoxia. In conclusion, the data demonstrate that growth conditions utilizing a xeno-free and hypoxic environment not only provide an improved environment for the expansion of ASCs, but also set the stage as a culture system with the potential broad-spectrum utility for regenerative medicine and tissue engineering applications.
The StemPro MSC SFM XenoFree +CELLstart culture system are commercial products. StemPro MSC SFM XenoFree culture medium is based on a proprietary formulation.https://www.liebertpub.com/doi/pdf/10.1089/ten.tec.2011.0592
|Source||Literature - modified commercial product|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > Yes||Yes|