|Cell line/type||ASCs (adipose tissue derived stem cells)|
|Product||StemPRO® MSC SFM XenoFree Patrikoski et al. 2013|
Patrikoski, M., Juntunen, M., Boucher, S., Campbell, A., Vemuri, M. C., Mannerström, B., & Miettinen, S. (2013). Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells. Stem cell research & therapy, 4(2), 27.
In this study, animal component-free isolation and cell-expansion protocols were developed for ASCs. ASCs cultured in XF/SF conditions had significantly higher proliferation rates compared with HS/FBS cultures. Characteristic immunophenotypes of ASCs were maintained in every condition; however, cells expanded in XF/SF conditions showed significantly lower expression of CD54 (intercellular adhesion molecule 1, ICAM-1) at low passage number. Further, multilineage differentiation potential of ASCs was maintained in every culture condition. The findings demonstrated that the novel XF/SF conditions maintained the basic stem cell features of ASCs and the animal-free workflow followed in this study has great potential in clinical cell therapies.
The precise formulation is proprietary. The adaptations to the commercial media are described.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707027/pdf/scrt175.pdf
|Source||Literature - modified commercial product|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > Yes||Yes|