Cell line/type | W9.5 ES cells |
---|---|
Species | Mouse |
Animal free | No |
Product | serum-free B4L |
Bruce, S. J., Gardiner, B. B., Burke, L. J., Gongora, M. M., Grimmond, S. M., & Perkins, A. C. (2007). Dynamic transcription programs during ES cell differentiation towards mesoderm in serum versus serum-free BMP4 culture. BMC genomics, 8(1), 365. DOI: 10.1186/1471-2164-8-365 The aims of this study were to determine the transcriptional programs associated with the stem cell state and to characterize mesoderm differentiation between serum and serum-free culture. ES cells were differentiated as embryoid bodies in 10% FBS or serum-free media containing BMP4 (2 ng/ml), and expression profiled using 47 K Illumina(R) Sentrix arrays. The media formulation of serum-freeB4L (defined serum-free media containing BMP4), is described in the Materials and Methods of Johansson et al. 1995. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |