Cell line/type | MDA-MB-231 (breast cancer cells, RRID:CVCL_0062) |
---|---|
Species | Human |
Animal free | No |
Product | MEM-oleate Przybytkowski et al. 2007 |
Przybytkowski, E., Joly, E., Nolan, C. J., Hardy, S., Francoeur, A. M., Langelier, Y., & Prentki, M. (2007). Upregulation of cellular triacylglycerol–free fatty acid cycling by oleate is associated with long-term serum-free survival of human breast cancer cells. Biochemistry and cell biology, 85(3), 301-310. In this study, the mechanism whereby oleate protects human breast cancer cells against apoptosis induced by serum withdrawal is studied. The metabolism of FFA, TG, and glucose, in parallel with long-term cell survival in the absence of serum, was investigated in a panel of human breast cancer cell lines and in nontransformed MCF-10A cells after treatment with exogenous oleate. Short-term (3-24 h) exposure of MDA-MB-231 human breast cancer cells to exogenous oleate resulted in a dose-dependent long-term (10 day) serum-free survival that correlated with the accumulation of TG in lipid droplets and with upregulation of lipolysis. Both effects persisted for several days after oleate removal. Rapid TG lipolysis and FFA re-esterification, supported by high rates of glycolysis that provide the glycerol backbone for TG synthesis, are consistent with the presence of very active TG-FFA cycling in human breast cancer cells. Only the cancer cell lines capable of accumulating TG showed long-term serum-free survival after oleate treatment. The results suggest that upregulation of TG-FFA cycling induced by oleate may be involved in maintenance of human breast cancer cell survival. The long-term serum-free survival in the human breast cancer cell lines was dependent on the dose of oleate and was associated with the formation of cytoplasmic lipid droplets and with increased TG content. Short-term oleate treatment promotes long-term serum-free cell survival The precise protocol of serum-starvation and short-term oleate treatment is described in the materials and methods, and in the results. http://go.galegroup.com/ps/i.do?v=2.1&it=r&sw=w&id=GALE%7CA176778877&prodId=AONE&userGroupName=utrecht |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |