Cell line/type | Cumulus-oocyte complexes |
---|---|
Species | Bovine (cow) |
Animal free | No |
Product | mSOF-ITS-BSA Goovaerts et al. 2012 |
Goovaerts, I. G. F., Leroy, J. L. M. R., Langbeen, A., Jorssen, E. P. A., Bosmans, E., & Bols, P. E. J. (2012). Unravelling the needs of singly in vitro-produced bovine embryos: from cumulus cell co-culture to semi-defined, oil-free culture conditions. Reproduction, Fertility and Development, 24(8), 1084-1092. In this study, the necessity and possible interactions of serum, cumulus cells, and oil during culture was investigated. Two new oil-free culture protocols were tested. When serum was used, co-culture with cumulus cells seems necessary, leading to consistently high blastocyst rates. Finally, a serum-free, oil-free culture system using insulin, transferrin, selenium and BSA resulted in embryos with similar total cell numbers and apoptotic cell ratios, but blastocyst rates did not equal those obtained with serum and co-culture. The formulation of the mSOF + ITS + BSA medium is listed in the Materials and Methods in Experiment 1: Progesterone production by a cumulus cell monolayer (formulation mSOF), and Experiment 4, concentration ITS and BSA. http://www.publish.csiro.au/rd/pdf/RD11286 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Semi-defined |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |